ncRNA basic information
ncRNA ID:
MI0000301
ncRNA Database:
miRBase
ncRNA Name:
miR-224
ncRNA Type:
miRNA
ncRNA Expression:
down-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
DESC1
ncRNA Pathway:
DESC1/EGFR/AKT pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
TUSC7, miR-224 and DESC1 expressions in ESCC tissues and cells were detected by qRT-PCR. Protein level of DESC1, EGFR and p-AKT were observed by Western blot. Overall survival was calculated using the Kaplan-Meier method. Dual-luciferase reporter gene assay and RIP assay were used to comfirm TUSC7 binding to miR-224, and miR-224 binding to DESC1. Cell proliferation, apoptosis, and colony formation was detected by MTT, Flow Cytometry and Colony formation assays.
Drug Response:
sensitive
Cancer basic information
Cancer:
esophageal squamous cell carcinoma
Tissue/Cell:
cell line (TE-13, KYSE140, EC9706, KYSE30,Het-1A)
Other information
Title:
LncRNA-TUSC7/miR-224 affected chemotherapy resistance of esophageal squamous cell carcinoma by competitively regulating DESC1.
Journal:
J Exp Clin Cancer Res
Published:
2018
PubMed ID:
29530057