ncRNA basic information
ncRNA ID:
ENSG00000259104
ncRNA Database:
Ensembl
ncRNA Name:
PTCSC3
ncRNA Type:
lncRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
INO80
ncRNA Pathway:
STAT3/INO80 pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00997 (APRD00185, DB05331, DB05847)
Drug Name:
Doxorubicin
Drug Method:
Expression of RNA and protein was analyzed by qRT-PCR and western blot, respectively. Flow cytometry was used to analyze the expression rate of CD133+ cells. The endogenous expression of related genes was modulated by recombinant plasmids and cell transfection. Combination condition and interaction between PTCSC3 and STAT3 were determined by RIP and RNA pull-down assay, respectively. MTT assay was performed to detect cytotoxicity. Chromatin immunoprecipitation was conducted to identify interactions between STAT3 and DNA promoter of INO80.
Drug Response:
sensitive
Cancer basic information
Cancer:
anaplastic thyroid cancer
Tissue/Cell:
cell line (8505C,FTC 238,FTC 133)
Other information
Title:
LncRNA PTCSC3 affects drug resistance of anaplastic thyroid cancer through STAT3/INO80 pathway.
Journal:
Cancer Biol Ther
Published:
2018
PubMed ID:
29561707