ncRNA basic information
ncRNA ID:
MI0000778
ncRNA Database:
miRBase
ncRNA Name:
miR-370
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR,Northern blot
ncRNA Target Gene:
ENG
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The expression levels of miRNA were determined by Quantitative RT-PCR and Northern blot . Those of protein were by Western blot analysis. Bioinformatics method was used to predict the target genes of miR-370. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs. Flow Cytometric Assay was used to determine if cells were viable, apoptotic, or necrotic. In vivo tumor xenograft studies was used to test the role of miR-370 .
Drug Response:
sensitive
Cancer basic information
Cancer:
endometrioid ovarian cancer
Tissue/Cell:
cell line (SKOV3, UWB1.289,HEY, OV2008,IGROV1 TOV112D,ES-2, TOV21G)
Other information
Title:
MicroRNA-370 suppresses proliferation and promotes endometrioid ovarian cancer chemosensitivity to cDDP by negatively regulating ENG.
Journal:
Cancer Lett
Published:
2014
PubMed ID:
25063739